Nuclear Factors lnteract with a Soybean ,&Conglycinin En hancer

Upstream sequences of the gene encoding the a‘ subunit of 8-conglycinin were analyzed for interactions with nuclear proteins from immature soybean seeds. Two factors were identified that interact with specific sequence elements within 257 base pairs 5‘ of the transcription start site. One factor, SEF 3, binds exclusively to a region composed of two elements located at -183 to -169 base pairs and -153 to -134 base pairs relative to the start of transcription. Each of these sites includes the hexanucleotide sequence AACCCA, which may serve as a primary recognition sequence. During seed development, SEF 3 binding activity was found to increase in soybean embryos during the time of 8-conglycinin synthesis and to decrease as seeds neared maturity. The position of the SEF 3 binding sequence corresponds with a previously reported seed-specific enhancer region, and it seems likely that this factor may act as a positive regulator of transcription of the 8-conglycinin, a‘ subunit gene in developing soybean seeds. The second factor, SEF 4, also binds within the -257 to -77 region but also interacts with sites located further upstream.